Analysis, Journal of Chromatography 82, 101-115 (1973).
Treiber, Utility of Thin-Layer Chromatography as an Analytical Tool. Journal of Chromatographic Science, 24, 220-224 (1986).
Ford-Holevinski, et al. Quantitation of Thin-Layer Chromatograms with an Apple II Computer-Based Videodensitometer. Analytical Biochemistry 150, 359-363 (1985).
Maniatas, et al. Molecular Cloning a Laboratory Man-
ual Cold Spring Harbor Laboratory p. 470 (1983).
Nagata, et al. Estimation of Lipid Concentrations on
Thin-Layer Plates by Densitometry of Transparent
Analytical Biochemistry 171, 248-255 (1988).
Steve Ciarcia; Build a Gray Scale Video Digitizer (Part
I and II); May 1987, Byte (pp. 95-138 Part I, pp.
129-138 Part II).
Primary Examiner—James J. Groody
Assistant Examiner—Michael H. Lee
Attorney, Agent, or Firm—Fulbright & Jaworski
The measurement of integrated densities of irregularly shaped areas of a subject specimen is accomplished by using a video camera to derive an analog video image signal representative of the optical intensity of light associated with the specimen. A modified low cost high speed video digitizer converts the video image signal into a digital format which an interactive computer program converts into digital optical density values that are used to determine the net integrated density of each irregularly shaped area within the specimen.
13 Claims, 21 Drawing Sheets