REACTION CASSETTE FOR PREFORMING SEQUENTIAL ANALYTICAL ASSAYS BY NONCENTRIFUGAL AND NONCAPILLARY MANIPULATIONS
 Inventors: Lowry J. Messenger, Granger, Ind.;
Christine D. Nelson, Edwardsburg,
Mich.; Kin-Fai Yip, Elkhart; Frank
W. Wogoman, Granger, both of Ind.
 Assignee: Miles Inc., Elkhart, Ind.
[ * ] Notice: The portion of the term of this patent subsequent to Feb. 5, 2008 has been disclaimed.
 Appl. No.: 774,362
 Filed: Oct. 8,1991
Related U.S. Application Data
 Continuation of Ser. No. 378,039, Jul. 11, 1989, abandoned, which is a continuation-in-part of Ser. No. 179,843, Apr. 11, 1988, Pat. No. 4,990,075.
 Int. CI.5 G01N 33/52; G01N 33/72
 U.S. CI 436/523; 436/67;
436/165; 436/501; 436/533; 436/808; 436/536; 435/299; 435/312; 422/58; 422/61; 422/72;
 Field of Search 422/55, 58, 61, 72,
422/102; 435/299, 312; 436/45, 17, 67,165, 501, 518, 522, 523, 526, 528, 533, 536, 807, 808
 References Cited
U.S. PATENT DOCUMENTS
4,883,763 11/1989 Holen 436/45
4,970,171 11/1990 Messenger et al 436/66
4,990,075 2/1991 Wogoman 422/58
Primary Examiner—James C. Housel
Assistant Examiner—David Redding
Attorney, Agent, or Firm—Andrew L. Klawitter
Analytical reaction cassette and method for performing sequential analytical assay procedures to determine the amount of an analyte in a liquid test mixture. The reaction cassette can be in the form of a substantially square container having a substantially horizontal axis of rotation and incorporated with one or more analytical reagents such that they are contacted with a liquid test mixture in a desired ordered sequence to perform a particular assay procedure. Corners provided by the substantially square configuration of the reaction cassette disrupt the flow of liquids disposed in the reaction cassette upon contact therewith to thereby agitate and mix such liquids. A liquid disposed in the reaction cassette is capable of being manipulated and mixed therein by rotating the reaction cassette about the horizontal axis at sufficiently low velocities wherein the transport of such liquid is noncentrifugal and due substantially only to gravitational force. The reaction cassette is particularly useful for performing immunoturbidimetric assays, e.g., for the determination of glycated hemoglobin.
57 Claims, 6 Drawing Sheets