(i9) United States
(12) Patent Application Publication oo) Pub. No.: US 2006/0188140 Al
Gholap et al. (43) Pub. Date: Aug. 24,2006
(54) METHOD AND SYSTEM FOR DIGITAL
IMAGE BASED TISSUE INDEPENDENT
SIMULTANEOUS NUCLEUS CYTOPLASM
AND MEMBRANE QUANTITATION
(75) Inventors: Abhijeet S. Gholap, Pune (IN); Gauri
A. Gholap, Pune (IN); Aparna Joshi,
Pune (IN); Amitabha Basu, Pune (IN);
C.V.K. Rao, Pune (IN); Prithviraj
Jadhav, Pune (IN)
Lesavich High-Tech Law Group, P.C.
39 S. LaSalle Street Chicago, IL 60603 (US)
(73) Assignee: Bioimagene, Inc., San Mateo, CA
(21) Appl. No.: 11/361,774
(22) Filed: Feb. 23, 2006
Related U.S. Application Data
(60) Provisional application No. 60/655,465, filed on Feb. 23, 2005.
(51) Int. CI.
G06K 9/00 (2006.01)
(52) U.S. CI 382/133
A method and system for automatic digital image based tissue independent simultaneous nucleus, cytoplasm and membrane quantitation. Plural types of pixels comprising cell components including at least cell cytoplasm and cell membranes from a biological tissue sample to which a chemical compound has been applied and has been processed to remove background pixels and pixels including counterstained components are simultaneously identified. The identified cell components pixels are automatically classified to determine a medical conclusion such as a human breast cancer, a human prostrate cancer or an animal cancer.