Method and system for digital image based tissue independent simultaneous ...

US 20060188140A1

(i9) United States

(12) Patent Application Publication oo) Pub. No.: US 2006/0188140 Al

Gholap et al. (43) Pub. Date: Aug. 24,2006

(54) METHOD AND SYSTEM FOR DIGITAL
IMAGE BASED TISSUE INDEPENDENT
SIMULTANEOUS NUCLEUS CYTOPLASM
AND MEMBRANE QUANTITATION

(75) Inventors: Abhijeet S. Gholap, Pune (IN); Gauri
A. Gholap, Pune (IN); Aparna Joshi,
Pune (IN); Amitabha Basu, Pune (IN);
C.V.K. Rao, Pune (IN); Prithviraj
Jadhav, Pune (IN)

Correspondence Address:

Lesavich High-Tech Law Group, P.C.

Suite 325

39 S. LaSalle Street Chicago, IL 60603 (US)

(73) Assignee: Bioimagene, Inc., San Mateo, CA

(21) Appl. No.: 11/361,774

(22) Filed: Feb. 23, 2006

Related U.S. Application Data

(60) Provisional application No. 60/655,465, filed on Feb. 23, 2005.

Publication Classification

(51) Int. CI.

G06K 9/00 (2006.01)

(52) U.S. CI 382/133

(57) ABSTRACT

A method and system for automatic digital image based tissue independent simultaneous nucleus, cytoplasm and membrane quantitation. Plural types of pixels comprising cell components including at least cell cytoplasm and cell membranes from a biological tissue sample to which a chemical compound has been applied and has been processed to remove background pixels and pixels including counterstained components are simultaneously identified. The identified cell components pixels are automatically classified to determine a medical conclusion such as a human breast cancer, a human prostrate cancer or an animal cancer.

FIG. 2

C START ^ /4

V'

AUTOMATICALLY ANALYZE LUMINANCE PARAMETERS
FROM A DIGITAL IMAGE OF A BIOLOGICAL SAMPLE TO
WHICH A CHEMICAL COMPOUND HAS BEEN APPLIED I 26

I

AUTOMATICALLY ANALYZE MORPHOLOGICAL PARAMETERS FROM INDIVIDUAL BIOLOGICAL COMPONENTS WITHIN THE BIOLOGICAL SAMPLE ON THE I 28

DIGITAL IMAGE

I

AUTOMATICALLY DETERMINE A MEDICAL CONCLUSION FROM THE ANALYZED LUMINANCE AND MORPHOLOGICAL

PARAMETERS I 30

^ END ^

IDENTIFY PLURAL AREAS OF INTEREST IN A DIGITAL
IMAGE OF A BIOLOGICAL TISSUE SAMPLE TO WHICH A
CHEMICAL COMPOUND HAS BEEN APPLIED

I

ELIMINATE PLURAL PIXELS COMPRISING A BACKGROUND
PORTION OF THE PLURAL AREAS OF INTEREST FROM THE
IDENTIFIED PLURAL AREAS OF INTEREST TO CREATE
MODIFIED PLURAL AREAS OF INTEREST

I

ELIMINATE PLURAL PIXELS COMPRISING COUNTERSTAINED COMPONENTS IN MODIFIED PLURAL AREAS OF INTEREST

I

SIMULTANEOUSLY IDENTIFY PLURAL PIXELS COMPRISING
CELL CYTOPLASM AND CELL MEMBRANES ARE IN THE
MODIFIED PLURAL AREAS OF INTEREST

i

CLASSIFY THE IDENTIFIED CELL COMPONENTS IN THE MODIFIED PLURAL AREAS OF INTEREST TO DETERMINE A MEDICAL CONCLUSION

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