United States Patent [19]
Kawasaki et al.
[11] Patent Number: 5,057,410 [45] Date of Patent: Oct. 15, 1991
[54] CHIMERIC MESSENGER RNA DETECTION METHODS
[75] Inventors: Ernest S. Kawasaki, Richmond;
Francis P. McCormick, Albany;
Owen O. Witto, Sherman Oaks, all of
Calif.
[73] Assignee: Cetus Corporation, Emeryville, Calif.
[21] Appl. No.: 229,604
[22] Filed: Aug. 5, 1988
[51] Int. C1.5 C12Q 1/68; C07H 15/12;
C12N 15/00
[52] U.S. CI 435/6; 536/27;
935/77; 935/78
[58] Field of Search 435/6; 536/27; 935/77,
935/78
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Primary Examiner—Robert A. Wax
Assistant Examiner—Mindy B. Fleisher
Attorney, Agent, or Firm—Stacey R. Sias; Richard M.
Snead; Kevin R. Kaster
[57] ABSTRACT
The invention provides highly sensitive methods for detecting specific sequences contained in chimeric mRNA. The mRNA sequences are reverse transcribed into complementary DNA (cDNA), amplified by the Polymerase Chain Reaction, and detected by hybridization with a labeled sequence specific oligonucleotide probe. The method is particularly valuable for the detection of chimeric mRNAs experessed by activated oncogenes that result from aberrant genetic rearragements such as chromosomal translocations.
24 Claims, 3 Drawing Sheets
