(12) United States Patent (10) Patent N0.: US 3,012,341 B2 Forrow et al. (45) Date of Patent: *Sep. 6, 2011 (54) BIOSENSOR ELECTRODE MEDIATORS FOR (56) References Cited REGENERATION OF COFACTORS U.S. PATENT DOCUMENTS (75) Inventors: Nigel J. Forrow, Oxon (GB); Gurdial S. Sanghera, Newton, MA (US); Jared L. Q 4 g:1:;nefta1a‘I' “““““““““ “ 205/792 WatkiI1,0Xf0rdShire(GB); Stephen 4,753,323 A 7/1933 Davis et 3.1. Walters, Binningham (GB) 4,946,562 A 8/1990 Guiuswaniy 5,096,669 A * 3/1992 Lauks et al. ........... .. 204/403.02 (73) Assignee: Abbott Laboratories, Alameda, CA 5,120,420 A 6/1992 Nankal at a1~ US) 5,126,034 A 6/1992 Carter et al. ( 5,212,622 A 5/1993 MacFarlane et al. . . . . . 5,266,179 A 11/1993 Nank ' tal. ( * ) Notice: Subject to any disclaimer, the term ofthis 5,512,159 A 4/1996 Yoshiilki et al‘ patent is extended or adjusted under 35 5,532,697 A 12/1996 Ikeda at 31‘ U.S.C. 154(b) by 878 days. 5,623,390 A 5/1997 Carteretal. . . . . . 5,651,869 A 7/1997 Yoshioka et al. This patent is subject to a tenninal dis- _ C1aimer_ (Continued) (21) App1_ NO_; 11/849,790 FOREIGN PATENT DOCUMENTS _ EP 0 125 867 11/1984 (22) Filed: Sep. 4, 2007 (Continued) (65) Prior Publication Data OTHER PUBLICATIONS US 2007/0289881 A1 Dec. 20, 2007 . . Wu et al, Analytical Chemistry 1996, 68, pp. 3688-3696* Related U.S. Application Data (Continued) (63) Continuation of application No. 10/832,408, filed on _ _ _ Apr. 26, 2004, HOW P61. N6. 7,504,019, which is a Prrrrrery Erarrrlrer — Kai K Olsen continuation of application No. 09/529,617, filed as (74) Attorney. /136111. 01’ Firm ’EdWaFd -I~ B303; Khin K~ application No. PCT/US98/21815 on Oct. 16, 1998, Chin; Bozicevic, Field & Francis, LLP now Pat. No. 6,736,957. 57 ABSTRACT (60) Provisional application No. 60/061,982, filed on Oct. ( ) _ _ _ _ + 16, 1997_ The present invention is based on the discovery of NAD and NADP+ mediator compounds that do not bind irreversibly to (51) Int. Cl. thiol groups in the active sites of intracellular dehydrogenase G01N 2 7/32 7 (2006-01) enzymes. Such mediator compounds avoid a common mode (52) U.S. Cl. .............. .. 205/777.5; 205/792; 204/403.04; of enzyme inhibition. The mediators can therefore increase 204/403-12 the stability and reliability of the electrical response in (58) Field of Classification Search ........... .. 204/403.01, amperometric electrodes constructed from NAD- or NADP204/403.04, 403.12, 403.14; 205/777.5, dependent enzymes. 205/792
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5,705,045 A 1/1998 Park 2002/0092612 Al 7/2002 Davies et al. 2004/0031682 Al 2/2004 Wilsey
EP 0 732 406 Al 9/1996
EP 0 794 429 9/1997
GB 2 188 728 10/1987
JP 07 209 243 8/1995
JP 7248310 9/1995
JP 8094573 4/1996
JP 8334489 12/1996
JP 9145665 6/1997
OTHER PUBLICATIONS
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