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Yanofsky et al. (1996), 'High Affinity type 1 interleukin 1 receptor antagonists discovered by screening recombinant peptide libraries', PNAS 93:7381-7386.

Yoshida et al. (1984), 'The Activity of Synthetic analogs of Serum
Thymic Factor (FTS) to Convert Mouse Pre-T Cells into Thy-1
Positive Cells', Int. J. Immunopharmac. 6(2):141-146.
Yu et al. (1994), 'Structural Basis for the Binding of Proline-Rich
Peptides to SH3 Domains', Cell 76:933-945.

Zheng et al. (1995), 'Administration of Noncytolytic IL-10/Fc in
Murine Models of Lipopolysaccharide-Induced Septic Shock and
Allogeneic Islet Transplantation', J. Immunol. 154:5590-5600.

Ishikawa et al (1998), 'GDlct-replica peptides functionally mimic GDI a, an adhesion molecule of metastatic tumor cells, and suppress the tumor metastasis', FEBS 441:20-24.

Kraftetal. (1999), 'DefinitionofanUnexpectedLigandRecognition Motif for ctv|36 Integrin', Journal of Biological Chemistry 274(4):1979-1985.

Maurer et al. (1997), 'Autodisplay: One-Component System for Efficient Surface Display and Release of Soluble Recombinant Proteins from Escherichia coli', ournal of Bacteriology 179(3):794-80. Russel et al, Introduction to phage biology and phage display. In A Practical Approach; Oxford University Press; 2004; 1-26.

* cited by examiner

FIG. 1

peptide selection

i

peptide optimization

i

formation of Fc-peptide DNA construct

4

insertion of construct into expression vector transfection of host cell with vector

4

expression of vector in host cell

4

Fc multimer formation in host cell

i

isolation of Fc multimer from host cell

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[blocks in formation]

FIG. 4

ATGGACAAAACTCACACATGTCCACCTTGTCCAGCTCCGGAACTCCTGGGGGGACCGTCA

TACCTGTTTTGAGTGTGTACAGGTGGAACAGGTCGAGGCCTTGAGGACCCCCCTGGCAGT

MDKTHTCPPCPAPELLGGPS

GTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTC

61 ++ + - - - + + +■ 120

CAGAAGGAGAAGGGGGGTTTTGGGTTCCTGTGGGAGTACTAGAGGGCCTGGGGACTCCAG

VFLFPPKPKDTLMISRTPEV

ACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTG

121 + + + + + + 180

TGTACGCACCACCACCTGCACTCGGTGCTTCTGGGACTCCAGTTCAAGTTGACCATGCAC

TCVVVDVSHEDPEVKFNWYV

GACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACG

181 + + + + + +• 240

CTGCCGCACCTCCACGTATTACGGTTCTGTTTCGGCGCCCTCCTCGTCATGTTGTCGTGC

DGVEVHNAKTKPRBEQYNST

TACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTAC

241 + + + -- + + ^ + 300

ATGGCACACCAGTCGCAGGAGTGGCAGGACGTGGTCCTGACCGACTTACCGTTCCTCATG

YRVVSVLTVLHQDWLNGKEY

AAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCC

301 + ♦ + + + + 360

TTCACGTTCCAGAGGTTGTTTCGGGAGGGTCGGGGGTAGCTCTTTTGGTAGAGGTTTCGG

KCKVSNKALPAPIEKTISKA

AAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACC

361 + + + -f + + 420

TTTCCCGTCGGGGCTCTTGGTGTCCACATGTGGGACGGGGGTAGGGCCCTACTCGACTGG

KGQPREPQVYTLPPSRDELT

AAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTG

421 + + + + + + 480

TTCTTGGTCCAGTCGGACTGGACGGACCAGTTTCCGAAGATAGGGTCGCTGTAGCGGCAC

KNQVSLTCLVKGFYPSDIAV

GAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGAC

481 ♦ + + ♦+ + 540

CTCACCCTCTCGTTACCCGTCGGCCTCTTGTTGATGTTCTGGTGCGGAGGGCACGACCTG

EWE3NGQPENNYKTTPPVLD

TCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAG

541 + + + + + + 600

AGGCTGCCGAGGAAGAAGGAGATGTCGTTCGAGTGGCACCTGTTCTCGTCCACCGTCGTC

SDGSFFLYSKLTVDKSRWQQ

GGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAG

601 + + ♦ ♦♦* 660

CCCTTGCAGAAGAGTACGAGGCACTACGTACTCCGAGACGTGTTGGTGATGTGCGTCTTC

GNVFSC3VMHEALHNHYTQK

AGCCTCTCCCTGTCTCCGGGTAAA

661 + + 684

TCGGAGAGGGACAGAGGCCCATTT

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