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Isolated nucleic acid moelcules encoding the Dw3 P-glycoprotein of sorghum ...

 Gurmukh S. Johal et al
The invention relates to the genetic modification of plants particularly to the expression of P-glycoprotein genes in transformed plants. Nucleotide sequences for Dw3 genes encoding a P-glycoprotein of sorghum, and method for their use are provided. The sequences fine use in modifying the growth...
Inventors: Gurmukh S. Johal, Dilbag S. Multani
Assignees: Pioneer Hi-Bred International , Inc., The Curators of the University of Missouri
Primary Examiner: Amy J. Nelson
Secondary Examiner: David H Kruse
Attorney: Alston & Bird LLP

U.S. Classification
800/298; 435/320.1; 435/419; 536/236; 800/320; 800/320.1; 800/320.2; 800/312; 800/290; 800/289

International Classification
C12N 1529; C12N 1582; A01H 500; A01H 510

View patent at USPTO

Claims

What is claimed is:

1. An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:

(a) the nucleotide sequence set forth in SEQ ID NO: 7;
(b) the nucleotide sequence set forth in SEQ ID NO: 8;
(c) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO: 9; and,
(d) a nucleotide sequence that is complemcentary to the nucleotide sequence of any one of (a)-(c).

2. An expression cassette comprising the nucleic acid molecule of claim 1, said nucleotide sequence operably linked to a promoter that drives expression in a plant cell.

3. The expression cassette of claim 2, wherein said promoter is selected from the group consisting of tissue-preferred, constitutive, chemically regulatable, and pathogen-inducible promoters.

4. A transformed plant having stably incorporated into its genome a nucleic acid molecule operably linked to a promoter that drives expression in a plant cell, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:

(a) the nucleotide sequence set forth in SEQ ID NO: 7;
(b) the nucleotide sequence set forth in SEQ ID NO: 8;
(c) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO: 9, and,
(d) a nucleotide sequence that is complementary to the nucleotide sequence of any one of (a)-(c).

5. The plant of claim 4, wherein said promoter is selected from the group consisting of tissue-preferred, constitutive, chemically regulatable, and pathogen-inducible promoters.

6. The plant of claim 4, wherein said nucleic acid molecule is operably linked to said promoter in the antisense orientation.

7. The plant of claim 4, wherein said plant is a monocot.

8. The plant of claim 7, wherein said monocot is selected from the group consisting of maize, wheat, rice, sorghum, rye, millet and barley.

9. The plant of claim 4, wherein said plant is a dicot.

10. The plant of claim 9, wherein said dicot is selected from the group consisting of soybeans, sunflowers, safflowers, alfalfa, Brassica sp., cotton, peanuts and fruit trees.

11. Transformed seed of the plant of claim 4.

12. Transformed seed of the plant of claim 5.

13. Transformed seed of the plant of claim 6.

14. Transformed seed of the plant of claim 7.

15. Transformed seed of the plant of claim 8.

16. Transformed seed of the plant of claim 9.

17. Transformed seed of the plant of claim 10.

18. A method for modifying the growth of a plant, said method comprising transforming a plant with a nucleic acid molecule encoding a P-glycoprotein, said nucleic acid molecule operably linked a promoter that drives expression of said nucleic acid molecule in said plant, said nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:

(a) the nucleotide sequence set forth in SEQ ID NO: 7;
(b) the nucleotide sequence set forth in SEQ ID NO: 8;
(c) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO: 9; and,
(d) a nucleotide sequence that is complementary to the nucleotide sequence of any one of (a)-(c).

19. The method of claim 18, wherein said nucleic molecule is operably linked to said promoter in the antisense orientation.

20. The method of claim 18, wherein the height of said plant is reduced.

21. The method of claim 18, wherein said plant is a monocot.

22. The method of claim 18, wherein said monocot is selected from the group consisting of maize, wheat, rice, sorghum, rye, millet and barley.

23. A transformed plant cell having stably incorporated into its genome a nucleic acid molecule operably linked to a promoter that drives expression in a plant cell, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:

(a) the nucleotide sequence set forth in SEQ ID NO: 7;
(b) the nucleotide sequence set forth in SEQ ID NO: 8;
(c) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO: 9; and,
(d) a nucleotide sequence that is complementary to the nucleotide sequence of any one of (a)-(c).