Separation of requirements for protein-DNA complex assembly from those for functional activity in the herpes simplex virus regulatory protein Vmw65

R Greaves, P O'hare - Journal of virology, 1989 - Am Soc Microbiol
A transient expression system was developed which results in efficient synthesis of the
regulatory protein Vmw65 of herpes simplex virus type 1 in eucaryotic cells. The gene for Vmw65 …

A cellular factor binds to the herpes simplex virus type 1 transactivator Vmw65 and is required for Vmw65-dependent protein-DNA complex assembly with Oct-1

P Xiao, JP Capone - Molecular and cellular biology, 1990 - Taylor & Francis
… transactivation domain but is fully capable of generating Vmw65-dependent complexes with
Oct-i and thus retains all the information necessary to direct protein-DNA complex assembly

[HTML][HTML] An unusual cellular factor potentiates protein-DNA complex assembly between Oct-1 and Vmw65.

GH Werstuck, JP Capone - Journal of Biological Chemistry, 1993 - Elsevier
… - Oct-1 Vmw65 Protein-DNA Complex Assembly tors equipped with various molecular weight
… of 1276 a Oct-1- Vmw65 Protein-DNA Complex Assembly bc to be stable for at least 30 min …

[HTML][HTML] Amino acid substitutions in the herpes simplex virus transactivator VP16 uncouple direct protein-protein interaction and DNA binding from complex assembly …

P Shaw, J Knez, JP Capone - Journal of Biological Chemistry, 1995 - ASBMB
The herpes simplex virus transactivator VP16 directs the assembly of a multicomponent
protein-DNA complex that requires the participation of two cellular factors, the POU …

Identification of a domain of the herpes simplex virus trans-activator Vmw65 required for protein-DNA complex formation through the use of protein A fusion proteins

G Werstuck, JP Capone - Journal of virology, 1989 - Am Soc Microbiol
… One of the host factors involved in this protein-DNA complex assembly has been identified
… of Vmw65 which is required for protein-DNA complex assembly. The procedure involved …

Kinetic Analysis of the Interaction of Mos1 Transposase with its Inverted Terminal Repeats Reveals New Insight into the ProteinDNA Complex Assembly

C Esnault, J Jaillet, N Delorme, N Bouchet… - …, 2015 - Wiley Online Library
Transposases are specific DNA‐binding proteins that promote the mobility of discrete DNA
segments. We used a combination of physicochemical approaches to describe the …

DNA-centered approaches to characterize regulatory protein–DNA interaction complexes

J Simicevic, B Deplancke - Molecular Biosystems, 2010 - pubs.rsc.org
Gene regulation is mediated by site-specific DNA-binding proteins or transcription factors (TFs),
which form protein complexes at regulatory loci either to activate or repress the …

Investigation of Protein-protein and Protein-DNA Interactions Involved in the Function of Herpes Simplex Virus Transactivator VMW65

XP Shaw - 1995 - macsphere.mcmaster.ca
The herpes simplex virus phosphoprotein Vmw65 (also referred to as VP16, αTIF), strongly
stimulates transcription through recognition of the cis-acting consensus sequence, …

[HTML][HTML] Structure of the TnsB transposase-DNA complex of type VK CRISPR-associated transposon

F Tenjo-Castaño, N Sofos, B López-Méndez… - Nature …, 2022 - nature.com
… suggests that the different conformation of the shTnsB1-2 and shTnsB3-4 protomers favours
an in trans architecture, most likely as a strategy to link the protein-DNA complex assembly

[HTML][HTML] Plant transformation by Agrobacterium tumefaciens: modulation of single-stranded DNA-VirE2 complex assembly by VirE1

D Frenkiel-Krispin, SG Wolf, S Albeck, T Unger… - Journal of biological …, 2007 - ASBMB
… The dissociation and protein-DNA complex assembly processes then proceeded in a
controlled manner that was promoted by macromolecular crowding effects. …