… DHO is a ubiquitous Zn metalloenzyme catalyzing the … of the pyrimidine ring– in the third
step of the de novo pyrimidine … molecule bridging Zn-α and Zn-β is displaced by the binding of …

… The pathway of biosynthesis of pyrimidine nucleoн tides de novo … twoto fivefold by anions
such as acetate, citrate, lactate, … is likely that two or three of these are involved in binding Zn 2 …

… The second gene required for de novo biosynthesis of UMP … of either the essential histidine
or a hydrated zinc atom (89). The … Generally these forms are determined by the anions in the …

… de novo pyrimidine biosynthesis pathway is conducted by DHO (EC 3.5.2.3) – a zinc
metalloenzyme … to affect substrate binding significantly; we assume that the substrate binding in …

… a zinc atom which is tightly bound … zinc atom at the active site. Although design of this
phosphinothioic acid incorporated multiple structural attributes which individually promote binding …

… phosphate mimic bound (PDB: 4GC3 ), products bound (PDB… formation of an alkoxide anion
intermediate that is initially … help inform efforts for de novo protein design and engineering of …

… between proteins in this superfamily we superimposed the … the residues involved in substrate
binding and catalysis in ASL. … purified using a Q-sepharose anion-exchange column (40 ml …

… one of the initial reactions for de novo biosynthesis of pyrimidine nucleotides: glutaminase-…
of a rare histidine with negative charge (histidinate anion) (Figure 3f). This third Zn 2+ ion has …

… controls the de novo biosynthesis of pyrimidines in animals, is … Furthermore, we found a
third Zn bound to a histidinate ion … in the rare form of a histidinate anion. Not surprisingly, given …

… Together these enzymes form a new superfamily of mononucleotide-binding domains. There
appears to be no other enzyme, however, which is composed of the same combination of …