| Publication number | CN100344224 C |
| Publication type | Grant |
| Application number | CN 200510048718 |
| Publication date | Oct 24, 2007 |
| Filing date | Dec 22, 2005 |
| Priority date | Dec 22, 2005 |
| Also published as | CN1799340A |
| Publication number | 200510048718.9, CN 100344224 C, CN 100344224C, CN 200510048718, CN-C-100344224, CN100344224 C, CN100344224C, CN200510048718, CN200510048718.9 |
| Inventors | 许继宏 |
| Applicant | 云南大学 |
| Export Citation | BiBTeX, EndNote, RefMan |
| Classifications (3), Legal Events (4) | |
| External Links: SIPO, Espacenet | |
技术领域 FIELD
本发明属植物种苗的生产技术领域,具体涉及通过组织培养大规模生产膏桐种苗的方法。 The present invention belongs to the field of production of plant seedlings, specifically relates to a method for mass production through tissue culture curcas seedlings.
背景技术 BACKGROUND
膏桐Jatropha curcas L.又名麻疯树、小桐子。 Jatropha Jatropha curcas L., also known as Jatropha, Jatropha. 分布于热带、亚热带地区。 Distributed in tropical and subtropical regions. 我国有栽培或半野生。 China has cultivated or semi-wild. 灌木或小乔木,高2-5m,多分枝。 Shrubs or small trees, high 2-5m, much branched. 速生。 Fast-growing. 喜肥沃土壤,酸性土或钙质土均宜生长。 Hi, fertile soil, acidic soil or calcareous soils are advised to grow. 耐0℃左右的低温及轻霜。 Resistance to low temperature of about 0 ℃ and light cream. 抗风力强。 Strong wind. 我国南海诸岛有分布。 South China Sea islands have distribution. 台、闽、粤、琼、桂、滇、黔、川等地栽培或半野生。 Taiwan, Fujian, Guangdong, Hainan, Guangxi, Yunnan, Guizhou, Sichuan and other cultivation or semi-wild. 多栽培作围篱及供观赏。 And more cultivated for ornamental fences. 种仁含油脂约50%,可作润滑油、制皂等用,并可催吐下泻,但有毒,忌食。 Seeds contain about 50% oil, can be used as lubricants, soap, etc. to use, and can induce vomiting tumble, but toxic, not eat.
实生苗3年生开始结实,可连年结果,正常结实期6-20年。 3-year-old seedlings start strong, can result in successive years, 6-20 years of normal seed. 结实间隔期不明显。 Strong interval obvious. 花单性,雌雄同株。 Flowers unisexual, monoecious. 二歧聚伞花序腋生。 Dichotomous cymes axillary. 雌花顶生于花序中央或二歧分叉处。 Born in the central or top female inflorescence dichotomous bifurcation. 雄花萼片和花瓣各5,花瓣长于花萼。 Male sepals and petals each 5 petals longer than calyx. 雄蕊10,2轮,内轮花丝合生,花盘腺体5。 Stamens 10,2 wheels, inner filaments connate, faceplate glands 5. 雌花花瓣短于花萼。 Female flower petals shorter than calyx. 子房上位,3室,每室1胚珠。 Ovary superior, 3 rooms, each room an ovule. 花柱3,箭形,基部合生,顶端2裂。 Styles 3, arrow, at base, the top 2 crack. 果实长2.5-4cm,径2-3cm,每果内有种子3粒,间有2粒,种子黑褐色至黑色,扁椭圆形,长1.6-2cm,宽1-1.3cm,有肉质胚乳,胚伸直,子叶宽扁,长椭圆形。 Fruit long 2.5-4cm, diameter 2-3cm, there are seeds per fruit 3, between two seeds dark brown to black, flat oval, long 1.6-2cm, width 1-1.3cm, fleshy endosperm, germ straight, wide and flat cotyledons, oblong.
现在栽培或半野生的膏桐繁殖方法只有种子繁殖和扦插繁殖,繁殖周期长,成本高。 Now cultivated or semi-wild breeding methods only curcas seed multiplication and cutting propagation, long reproductive cycle, high cost. 植物组织培养,就是分离植物体的一部分组织,如根、茎段、叶、花、幼胚等,在无菌试管中,配合一定的营养、激素、温度、光照等条件,使其生成完整植株。 Plant tissue culture is part of the organizational separation plants, such as roots, stems, leaves, flowers, and other immature embryos, in a sterile test tube, with certain nutrients, hormones, temperature, light and other conditions to generate a complete plant . 由于其条件可以严格控制,生长迅速,1个月左右即为一个周期,因此组织培养被称为快速繁殖,膏桐若能应用组织培养进行快速繁殖,将在膏桐种苗生产上有重要应用价值,但至今没有膏桐应用组织培养进行快速繁殖的报道和使用。 Conditions can be strictly controlled because of its rapid growth, a month or so that is a cycle, and therefore is called the rapid propagation of tissue culture, tissue culture curcas if rapid propagation, will have important applications in curcas seed production value, but so far no curcas application coverage and use of tissue culture and rapid propagation of.
发明内容 SUMMARY
本发明的目的在于提供通过组织培养大规模生产膏桐种苗的方法,为膏桐的人工繁殖、脱毒苗生产、培育优良品种开辟高效的途径。 The purpose of the present invention is to provide a large-scale production by tissue culture methods curcas seedlings for artificial propagation of Jatropha curcas, virus-free seedling production, cultivation of improved varieties open and efficient way.
本发明方法包括以下步骤:1、取膏桐茎尖,经消毒灭菌后,接种于加有20%~40%重量的玉米淀粉的如下培养基:MS+6-BA 0.5~1.5mg/L+NAA 0.2~2mg/L+糖20~40g/L,用3~5%琼脂固化,进行光照培养,PH值5.8-6、培养室温度26±2℃、光照强度1000-2000LX,光照时间每天10~14小时,培养20~28天,形成愈伤组织。 The method comprises the following steps: 1. Take curcas shoot tip, after sterilization, inoculated in the following medium plus 20% to 40% by weight of corn starch: MS + 6-BA 0.5 ~ 1.5mg / L + NAA 0.2 ~ 2mg / L + sugar 20 ~ 40g / L, with 3 to 5% agar solidified, make light culture, PH value of 5.8-6, training room temperature 26 ± 2 ℃, light intensity 1000-2000LX, illumination time 10 day to 14 hours, and cultured for 20 to 28 days to form a callus.
2、将愈伤组织切割后转到同样培养基上进行继代增殖培养,生成膏桐组培芽。 2. After cutting the callus were transferred on the same medium subculture, generate curcas tissue culture buds.
3、将多周期大量增殖的膏桐组培芽接入如下生根培养基培养以获得组织培养苗:IBA0.3~0.6mg/L+MS,经10~20天生根。 3, the multi-cycle proliferated tissue shoots of Jatropha curcas as rooting medium for access in order to obtain tissue culture seedlings: IBA0.3 ~ 0.6mg / L + MS, after 10 to 20 days rooted.
4、将组织培养苗出瓶栽培,出瓶栽培后的组织培养苗先保温保湿15-20天,保持温度不低于摄氏17度。 4, the tissue culture planting seedlings out of the bottle, tissue culture planting seedlings out of the bottle after the first 15-20 days of holding moisture, keeping the temperature of not less than 17 degrees Celsius. 可用塑料膜大棚或覆盖距地面20-40cm的塑料膜进行苗上保湿。 Available greenhouse plastic film or plastic film covering 20-40cm from the ground was the seedling moisture. 本发明方法生成的膏桐组织培养苗可直接移栽至苗圃地,不需要在苗床上进行练苗。 The method of the present invention is produced in tissue culture curcas seedlings can be transplanted directly to the nursery, seedlings do not need to practice in the seedbed.
本发明的有益效果:解决了膏桐应用组织培养进行快速繁殖的问题,周期短、成本低,完全能满足膏桐大面积栽培的需要,为膏桐的人工繁殖、脱毒苗生产、培育优良品种提供了一种高效的途径,在膏桐的开发利用中,具有较好的应用前景。 The beneficial effects of the invention: curcas application solves the problem by tissue culture and rapid propagation of short cycle, low cost, fully able to meet the needs of large-scale cultivation of Jatropha, Jatropha curcas for artificial breeding, virus-free seedling production, cultivate good Variety provides an efficient way in the development and utilization of Jatropha curcas, has good application prospects.
具体实施方式 DETAILED DESCRIPTION
见如下实施例:取膏桐茎尖,用洁净水清洗干净后,在流水下冲洗10min,然后转到洁净工作台上,吸干水分,先用70%酒精灭菌30s,再转入0.1%的HgCl2溶液中灭菌6min,无菌蒸馏水冲洗5次,茎尖接种于加有30%重量的玉米淀粉的如下培养基:MS+6-BA0.5mg/L+NAA 2mg/L+蔗糖30g/L,用4%的琼脂固化,光照培养,pH调为6,温度25±1℃,光照强度1500LX,光照时间每天12小时。 See the following examples: Take curcas shoot tip, wash with clean water, rinse under running water 10min, then go to clean the table, dry water, first with 70% alcohol sterilization 30s, and then transferred to 0.1% The HgCl2 solution was sterilized 6min, rinsed five times in sterile distilled water, shoot tips were inoculated in a medium added with 30% by weight of the following maize starch: MS + 6-BA0.5mg / L + NAA 2mg / L + sucrose 30g / L with 4% agar solidified, light culture, pH was adjusted to 6, the temperature 25 ± 1 ℃, light intensity 1500LX, illumination time 12 hours a day. 外植体接种后一周左右即可见明显膨大,形成直径约1cm左右的愈伤组织。 Explants about one week after inoculation can see obvious enlargement, with a diameter of about 1cm around the callus. 将愈伤组织切割后转到同样培养基上进行继代增殖培养。 After cutting the callus were transferred on the same medium subculture. 用多周期大量增殖的膏桐组培芽生产种苗,生根用培养基为IBA0.3mg/L+MS,经14天生根。 Multi-cycle proliferated tissue culture buds of Jatropha curcas seed production, rooting medium was used IBA0.3mg / L + MS, after 14 days rooted. 当根长0.5cm左右,叶生长到3-5片时,将生根种苗直接移栽至苗圃地,先保温保湿15天。 When the root length of about 0.5cm, the leaves grow to 3-5 films, will be directly transplanted to nursery seedling roots, the first 15 days holding moisture. 保持温度不低于摄氏17度,用塑料膜大棚进行苗上保湿。 Keep the temperature below 17 degrees Celsius, with a plastic film greenhouses were on seedling moisture. 随后苗木生长良好。 Then the seedlings grow well.
以上实施例仅对发明做进一步的说明,而本发明的范围不受所举实施例的局限。 Example only further embodiment of the invention described above, and the scope of the present invention is not confined to the illustrated embodiment.
| Date | Code | Event | Description |
|---|---|---|---|
| Jul 12, 2006 | C06 | Publication | |
| Sep 6, 2006 | C10 | Request of examination as to substance | |
| Oct 24, 2007 | C14 | Granted | |
| Feb 17, 2010 | C19 | Lapse of patent right due to non-payment of the annual fee |