一种小桐子专用生长调节剂及其应用 A small, dedicated Jatropha growth regulator and its application

技术领域 Technical Field

[0001] 本发明属于植物生长调控技术领域，具体涉及一种小桐子专用生长调节剂。 [0001] The present invention belongs to the technical field of plant growth regulation, in particular to a Jatropha special growth regulators. 同时，本发明还涉及该生长调节剂的具体应用。 Also, the present invention also relates to the growth regulator application.

背景技术 Background

[0002] 小桐子(Jatropha curcas L.)又名小油桐、麻疯树(麻风树)、膏桐等，属于大戟科(Euphorbiaceae)麻风树属(Jatropha)的灌木或小乔木。 [0002] Jatropha (Jatropha curcas L.), also known as Jatropha, Jatropha (Jatropha), Jatropha, etc., are Euphorbiaceae (Euphorbiaceae) Jatropha (Jatropha) shrubs or small trees. 高2〜5米,具有水状液汁，树皮平滑；枝条苍灰色，无毛，疏生突起皮孔，髓部大。 2~5 meters high, with a watery sap, bark smooth; branches pale gray, hairless, sparsely projections Picon, pith large. 叶纸质，近圆形至卵圆形，长7〜18厘米，宽6〜16厘米，顶端短尖，基部心形，全缘或3〜5浅裂，上面亮绿色，无毛，下面灰绿色，初沿脉被微软柔毛，后变无毛；掌状脉5〜7 ;叶柄长6〜18厘米；托叶小。 Leaves papery, nearly round to oval, long 7~18 cm wide and 6~16 cm, apex mucronate, base heart-shaped, entire or three to five lobed, bright green above, hairless, gray below green, beginning by Microsoft pubescent, later glabrescent along veins; 5 ~ 7 palmate veins; petiole 6~18 cm; stipules. 花序腋生，长6〜10厘米，苞片披针形，长4〜8毫米；花单性同株，聚伞花序腋生，通常雌花生长于花序中心位置，数朵雄花包围雌花。 Inflorescence axillary, 6~10 cm long, bracts lanceolate, 4 to 8 mm long; flowers unisexual same strain, cymes axillary, usually female flowers grow in inflorescences central location, surrounded by several male flowers female flowers. 雄花:雄蕊10，排成2轮，外轮5枚分离，内轮5枚花丝基部合生。 Male flowers: stamens 10, arranged in two, five separate outer, inner wheel 5 filaments basally connate. 雌花:子房3室，无毛，顶端2裂。 Female flowers: ovary Room 3, hairless, top 2-lobed. 蒴果类球形，直径·长2.5〜3厘米，黄色。 Capsule spherical, the diameter and length 2.5~3 cm, yellow. 种子椭圆状，长径约1.5〜2厘米，黑色(据《中国植物志》)。 Seeds oval, long diameter of about 1.5~2 cm, black (according to "Flora of China"). 小桐子广泛分布于热带和亚热带地区，可以在干旱贫瘠的地方生长，是一种多用途的树种。 Jatropha is widely distributed in tropical and subtropical regions, can be grown in arid areas, it is a versatile species. 其种子含油率一般在30〜40%，在生物能源利用方面潜力巨大。 Its seed oil content is generally 30 to 40%, a huge potential in terms of bio-energy use.

[0003] 小桐子是雌雄同株异花植物，但雌花的比例很低，约为3%〜7%，因此每个花序最终结果的数量一般仅为10个左右。 [0003] The Jatropha plant is monoecious, but the proportion of female is very low, about 3% ~ 7%, the number of each inflorescence end result is typically only 10 or so. 由于果实数量少，导致小桐子种子的产量无法满足生物柴油产业发展对原料的大量需求。 Due to a small number of fruit, resulting Jatropha seed production can not meet biodiesel industry a lot of demand for raw materials. 因此，如何提高小桐子的雌花比例和雌花总量就成为本领域技术研发的主攻方向。 Therefore, how to improve the proportion of female and total female Jatropha has become the main direction of the art research and development. 一般的做法是通过筛选优良单株进行无性繁殖，以期得到雌花比例高、结果数量多的新型品种，但实际效果并不理想。 The general practice is an excellent screening plant through asexual reproduction, in order to get a high proportion of female flowers, the result more than the number of new species, but the actual effect is not ideal. 而运用植物生长调节剂处理小桐子植株，以增加其总花数、雌花与两性花比例、果实数量，最终提升种子产量的方法，在现有技术中尚未见有相关报道。 And the use of plant growth regulators on Jatropha plant to increase its total number of flowers, the proportion of female and hermaphrodite flowers, the number of fruits, and ultimately enhance the seed production, in the prior art have not been reported.

发明内容 DISCLOSURE

[0004] 本发明的目的在于针对现有技术的不足，提供一种小桐子专用生长调节剂，以提高小桐子种子产量，满足市场对原料的迫切需求。 [0004] The object of the present invention is for the deficiencies of the prior art and to provide a dedicated Jatropha growth regulators to improve Jatropha seed production to meet the urgent market demand for raw materials.

[0005] 本发明的目的还在于提供一种使用该生长调节剂控制小桐子植株生长发育的具体方法。 [0005] The object of the present invention is to provide a use of the growth regulator Jatropha specific method of controlling plant growth and development.

[0006] 本发明的目的通过以下技术方案予以实现。 [0006] The object of the present invention will be realized by the following technical solutions.

[0007] 除非另有说明，本发明所采用的百分数均为重量百分数。 [0007] Unless otherwise stated, the percentages used in the present invention are weight percentages.

[0008] 一种小桐子专用生长调节剂，由下述方法制成:将细胞分裂素溶解于氢氧化钠溶液里，再用蒸馏水稀释成细胞分裂素浓度为0.02mg/mL〜100mg/mL的母液；将2〜9999份母液、I〜10份表面活性剂与O〜9997份蒸馏水混合均匀，得到所需的生长调节剂。 [0008] A Jatropha special growth regulators, is made by the following method: the cytokinin dissolved in sodium hydroxide solution, the diluted with distilled water to a cytokinin concentration 0.02mg / mL~100mg / mL of The mother liquor; 2~9999 parts of the mother liquor, I~10 parts surfactant O~9997 parts distilled water were mixed uniformly, to give the desired growth regulator.

[0009] 所述的细胞分裂素选自6-节基氨基嘌呤(6-benzylaminopurine, 6-BA)、激动素(kinetin,KT)、2_ 异戍烯腺嘌呤(2-1sopentennyladenine, 2_iP)、卩比效隆(N-(2-chloro_4-pyridyl)-N' phenylurea, CPPU)、噻重氮苯基服(Thidiazuron, TDZ)和I, 3-二苯基服(N,N' -diphenylurea)中的一种。 [0009] The cytokinin is selected from 6-membered aminopurine (6-benzylaminopurine, 6-BA), kinetin (kinetin, KT), 2_ iso-pentene adenine (2-1sopentennyladenine, 2_iP), Jie Lung efficiency ratio (N- (2-chloro_4-pyridyl) -N 'phenylurea, CPPU), thiadiazole phenyl clothes (Thidiazuron, TDZ) and I, 3- diphenyl clothing (N, N' -diphenylurea) in a.

[0010] 所述的细胞分裂素优选6-苄基氨基嘌呤。 [0010] Preferably said cytokinin 6-benzyl-aminopurine.

[0011] 所述的表面活性剂选自吐温20 (Tween-20)、吐温80 (Tween-80)、曲拉通X-100 (Triton X-100)、十二烧基硫酸钠(又称月桂醇硫酸钠，Sodiumdodecyl sulfate,缩写为SDS)、十二烧基苯横酸纳(Sodium dodecylbenzenesulfonate,缩写为SDBS)中的一种。 [0011], wherein the surfactant is selected from Tween 20 (Tween-20), Tween 80 (Tween-80), Triton X-100 (Triton X-100), twelve burning alkyl sulfate (also called sodium lauryl sulfate, Sodiumdodecyl sulfate, abbreviated as SDS), twelve cross burning benzene acid sodium (Sodium dodecylbenzenesulfonate, abbreviated as SDBS) in one.

[0012] 所述的表面活性剂优选吐温20。 [0012] The surfactant is preferably Tween 20.

[0013] 所述的小桐子专用生长调节剂的使用方法:于小桐子花芽出现前后10天内，将该生长调节剂均匀喷雾于小桐子全株直至有液滴滴下，每个开花期喷施I〜3次，2次喷施之间应间隔一天。 [0013] The use of the Jatropha special growth regulators: Jatropha flower buds appear on the front and rear 10 days, the growth regulator uniform spray in the whole plant Jatropha until a droplet dripping, spraying each flowering stage I An interval between ~ 3 times, 2 times a day spraying.

[0014] 与现有技术相比，本发明具有以下优点:已知细胞分裂素具有促进细胞分裂、诱导芽的形成并促进其生长的功能。 [0014] Compared with the prior art, the invention has the following advantages: known cytokinins promote cell division and induce bud formation and promote its growth capabilities. 以往大多数研究是关于细胞分裂素对植物的营养生长的影响，但至今未见有研究细胞分裂素对小桐子开花结果和种子含油量的影响的报道。 Most previous studies about the effect of cytokinin on the vegetative growth of the plants, but so far no studies have cytokinin on Jatropha flowering and seed oil content of the impact reports. 本发明以细胞分裂素为主要原料制成小桐子专用生长调节剂，小桐子植株经该生长调节剂喷施处理后，产生的总花数、雌花比例、果实数量和种子产量分别是未处理小桐子的3.6倍、4.3倍、4.5倍和3.9倍。 The present invention as the main raw material in cytokinin Jatropha special growth regulators, after Jatropha plant growth regulator by the spraying process, the total number of flowers, the female proportion of the number of fruit and seed yield were produced by small untreated Kiriko 3.6 times, 4.3 times, 4.5 times and 3.9 times. 同时，种子含油率也由31.67%提高到34.76%，增加了9.8%左右。 Meanwhile, seed oil content also increased from 31.67% to 34.76%, an increase of about 9.8%. 作为优选的6-节氨基嘌呤(6-Benzylaminopurine)是一种人工合成的细胞分裂素,具有稳定、高效、廉价和易于使用等特点。 Preferred 6-sections aminopurine (6-Benzylaminopurine) is a synthetic cytokinin, a stable, efficient, inexpensive and easy-to-use features. 本发明操作简便，成本低廉，经济效益显著，具有很好的应用前景。 The present invention is simple, low cost and significant economic benefits, has good prospects.

附图说明 Brief Description

[0015] 图1不同浓度的小桐子专用生长调节剂处理后的小桐子总花数的比较； Compare the total number of flowers of Jatropha specialty growth regulator treatment [0015] FIG. 1 with different concentrations of Jatropha;

[0016] **表示处理与对照相比，差异极显著(在0.01水平上)。 [0016] ** indicates treatment compared with the control, the difference was significant (at the 0.01 level).

[0017] 图2不同浓度的小桐子专用生长调节剂处理后的小桐子不同性别类型的花所占比例的比较； [0017] Comparison of different sex types of Jatropha flowers proportion specialty growth regulators Figure 2 different concentrations of Jatropha;

[0018] **表示处理与对照相比，差异极显著(在0.01水平上)。 [0018] ** indicates treatment compared with the control, the difference was significant (at the 0.01 level).

[0019] 图3不同浓度的小桐子专用生长调节剂处理后的小桐子结果情况与未处理的对照比较； [0019] FIG. 3 different concentrations of Jatropha Jatropha result of special circumstances after growth regulator treatment compared with untreated controls;

[0020] 图4不同浓度的小桐子专用生长调节剂处理后的小桐子结果数量的比较； [0020] The relatively small number of results after Jatropha 4 different concentrations of Jatropha dedicated growth regulator treatment;

[0021] **表示处理与对照相比，差异极显著(在0.01水平上)。 [0021] ** indicates treatment compared with the control, the difference was significant (at the 0.01 level).

[0022] 图5不同浓度的小桐子专用生长调节剂处理后的小桐子每个果实中种子数及单粒种子重量的比较； [0022] Figure 5 different concentrations of Jatropha special growth regulators compare Jatropha seeds and single seed weight per fruit;

[0023] **表示处理与对照相比，差异极显著(在0.01水平上)。 [0023] ** indicates treatment compared with the control, the difference was significant (at the 0.01 level).

[0024] NS表示处理与对照相比，差异不显著(在0.05水平上)。 [0024] NS indicates treatment compared with the control, the difference was not significant (at the 0.05 level).

[0025] 图6不同浓度的小桐子专用生长调节剂处理后的小桐子种子含油量的比较。 [0025] Figure 6 Comparison of different concentrations of Jatropha special growth regulators Jatropha seed oil content.

[0026] **表示处理与对照相比，差异极显著(在0.01水平上)。 [0026] ** indicates treatment compared with the control, the difference was significant (at the 0.01 level).

[0027] NS表示处理与对照相比，差异不显著(在0.05水平上)。 [0027] NS indicates treatment compared with the control, the difference was not significant (at the 0.05 level). 具体实施方式 DETAILED DESCRIPTION

[0028] 下面结合附图和实施例，对本发明作进一步地详细说明。 [0028] The accompanying drawings and the following embodiments, the present invention will be described in detail further. 但它们不是对本发明的限定。 But they are not limiting the present invention.

[0029] 实施例1 [0029] Example 1

[0030] 制备小桐子专用生长调节剂。 [0030] Preparation of Jatropha special growth regulators. 先将I克6-苄氨基嘌呤溶解于5毫升的IM的氢氧化钠溶液里，再用蒸馏水稀释成25mg/mL的母液，将16mL母液、IOmL吐温20与19.974L蒸馏水混合均匀，得到6-苄氨基嘌呤浓度为20mg/L的小桐子专用生长调节剂。 First I g of 6-benzyl aminopurine was dissolved in 5 ml of sodium hydroxide solution in the IM, was diluted with distilled water to 25mg / mL of the mother liquor, the mother liquor 16mL, IOmL 19.974L Tween 20 and distilled water were mixed uniformly to obtain 6 - benzylaminopurine concentration of 20mg / L of Jatropha special growth regulators.

[0031] 实施例2 [0031] Example 2

[0032] 重复实施例1，但有以下不同点:将32mL母液、IOmL吐温20与19.958L蒸馏水混合均匀，得到6-苄氨基嘌呤浓度为40mg/L的小桐子专用生长调节剂。 [0032] Example 1 was repeated, but with the following differences: A 32mL liquor, IOmL 19.958L Tween 20 and distilled water were mixed uniformly to give 6-benzylaminopurine concentration of 40mg / L of Jatropha special growth regulators.

[0033] 实施例3 [0033] Example 3

[0034] 重复实施例1，但有以下不同点:将64mL母液、IOmL吐温20与19.926L蒸馏水混合均匀，得到6-苄氨基嘌呤浓度为80mg/L的小桐子专用生长调节剂。 [0034] Example 1 was repeated, but with the following differences: A 64mL liquor, IOmL 19.926L Tween 20 and distilled water were mixed uniformly to give 6-benzylaminopurine concentration of 80mg / L of Jatropha special growth regulators.

[0035] 实施例4 [0035] Example 4

[0036] 重复实施例1，但有以下不同点:将128mL母液、IOmL吐温20与19.862L蒸馏水混合均匀，得到6-苄氨基嘌呤浓度为160mg/L的小桐子专用生长调节剂。 [0036] Example 1 was repeated, but with the following differences: A 128mL liquor, IOmL 19.862L Tween 20 and distilled water were mixed uniformly to give 6-benzylaminopurine concentration of 160mg / L of Jatropha special growth regulators.

[0037] 实施例5 [0037] Example 5

[0038] 重复实施例1，但有以`下不同点:将256mL母液、IOmL吐温20与19.734mL蒸馏水混合均匀，得到6-苄氨基嘌呤浓度为320mg/L的小桐子专用生长调节剂。 [0038] Example 1 was repeated, but with `different points: the 256mL liquor, IOmL 19.734mL Tween 20 and distilled water mixed to give 6-benzylaminopurine concentration of 320mg / L of Jatropha special growth regulators.

[0039] 实施例6 [0039] Example 6

[0040] 重复实施例1，但有以下不同点:将384mL母液、IOmL吐温20与19.606L蒸馏水混合均匀，得到6-苄氨基嘌呤浓度为480mg/L的小桐子专用生长调节剂。 [0040] Example 1 was repeated, but with the following differences: A 384mL liquor, IOmL 19.606L Tween 20 and distilled water were mixed uniformly to give 6-benzylaminopurine concentration of 480mg / L of Jatropha special growth regulators.

[0041] 实施例7〜12 [0041] Example 7~12

[0042] 重复实施例1〜6，但有以下不同点:所用的生长调节剂为激动素。 [0042] Example 1~6 was repeated, but with the following differences: the use of growth regulator Kinetin.

[0043] 实施例13〜18 [0043] Example 13~18

[0044] 重复实施例1〜6，但有以下不同点:所用的生长调节剂为2-异戊烯腺嘌呤。 [0044] Example 1~6 was repeated, but with the following differences: the use of growth regulators is 2 isopentenyladenine.

[0045] 实施例19〜24 [0045] Example 19~24

[0046] 重复实施例1〜6，但有以下不同点:所用的生长调节剂为吡效隆。 [0046] Example 1~6 was repeated, but with the following differences: the use of growth regulators to topiramate effect long.

[0047] 实施例25〜30 [0047] Example 25~30

[0048] 重复实施例1〜6，但有以下不同点:所用的生长调节剂为噻重氮苯基脲。 [0048] Example 1~6 was repeated, but with the following differences: the use of growth regulators for the thiadiazole phenyl urea.

[0049] 实施例31〜36 [0049] Example 31~36

[0050] 重复实施例1〜6，但有以下不同点:所用的生长调节剂为1，3_ 二苯基脲。 [0050] Example 1~6 was repeated, but with the following differences: the use of growth regulators to 1,3_ diphenyl urea.

[0051] 实施例37〜42 [0051] Example 37~42

[0052] 重复实施例1〜6，但有以下不同点:所用的表面活性剂为吐温80。 [0052] Example 1~6 was repeated, but with the following differences: the surfactant used was Tween 80.

[0053] 实施例43〜48 [0053] Example 43~48

[0054] 重复实施例1〜6，但有以下不同点:所用的表面活性剂为曲拉通X-100。 [0054] Example 1~6 was repeated, but with the following differences: the surfactant used is Triton X-100.

[0055] 实施例49〜54 [0055] Example 49~54

[0056] 重复实施例1〜6，但有以下不同点:所用的表面活性剂为十二烷基硫酸钠。 [0056] Example 1~6 was repeated, but with the following differences: the surfactant used was sodium dodecyl sulfate.

[0057] 实施例55〜60[0058] 重复实施例1〜6，但有以下不同点:所用的表面活性剂为十二烷基苯磺酸钠。 [0057] Example 55~60 [0058] Example 1~6 was repeated, but with the following differences: the surfactant used is sodium dodecyl benzene sulfonate.

[0059] 应用实施例1 [0059] Application Example 1

[0060] 选择花期前10天内的小桐子，此时小桐子花芽已开始分化但尚未可见。 [0060] selection 10 days before flowering Jatropha, Jatropha flower buds at this time has begun to differentiate but not yet visible. 在喷施前按实施例1所述方法配制好生长调节剂，于上午叶片上没有露水后均匀喷雾，将生长调节剂喷施于整个植株直至有液滴滴下为止。 Before spraying by the method described in Example 1 preparation of a good growth regulators, there is no uniform spray in the morning dew on the leaves after the growth regulator sprayed on the whole plant up until a droplet dripping. 共喷施3次，每次间隔一天。 Spraying total 3 times a day interval.

[0061] 应用实施例2 [0061] Application Example 2

[0062] 重复应用实施例1，有以下不同点:选择处于始花期的小桐子进行喷施，所述的始花期是指小桐子产生花芽后10天内。 [0062] Application Example 1 was repeated with the following differences: select in the first flowering of Jatropha were sprayed, said flowering means Jatropha produce 10 days after the buds.

[0063] 应用实施例3〜4 [0063] Application Example 3 to 4

[0064] 重复应用实施例1和2，有以下不同点:喷施实施例2所得生长调节剂(含40mg/L 6-苄氨基嘌呤)。 [0064] Duplicate Application Examples 1 and 2, the following point: Example 2 was sprayed with growth regulators (containing 40mg / L 6- benzylaminopurine).

[0065] 应用实施例5〜6 [0065] Application Example 5 to 6

[0066] 重复应用实施例1和2，有以下不同点:喷施实施例3所得生长调节剂(含80mg/L 6-苄氨基嘌呤)。 [0066] Duplicate Application Examples 1 and 2, the following point: obtained in Example 3 Spraying growth regulators (including 80mg / L 6- benzylaminopurine).

[0067] 应用实施例7〜8 [0067] Application Example 7~8

[0068] 重复应用实施例1和2，有以下不同点:喷施实施例4所得生长调节剂(含160mg/L 6-苄氨基嘌呤)。 [0068] Duplicate Application Examples 1 and 2, the following point: Example 4 obtained in Example spraying growth regulators (containing 160mg / L 6- benzylaminopurine).

[0069] 应用实施例9〜10 [0069] Application Example 9~10

[0070] 重复应用实施例1和2，有以下不同点:喷施实施例5所得生长调节剂(含320mg/L 6-苄氨基嘌呤)。 [0070] Duplicate Application Examples 1 and 2, the following point: Spraying obtained in the example 5 growth regulators (including 320mg / L 6- benzylaminopurine).

[0071] 应用实施例11〜12 [0071] Application Example 11~12

[0072] 重复应用实施例1和2，有以下不同点:喷施实施例6所得生长调节剂(含480mg/L 6-苄氨基嘌呤)。 [0072] Duplicate Application Examples 1 and 2, the following point: Example 6 obtained in Example spraying growth regulators (including 480mg / L 6- benzylaminopurine).

[0073] 应用实施例13〜24 [0073] Application Example 13~24

[0074] 重复应用实施例1〜12，有以下不同点:共喷施两次，每次间隔一天。 [0074] Application Example 1~12 repeated with the following differences: total sprayed twice a day intervals.

[0075] 应用实施例25〜36 [0075] Application Example 25~36

[0076] 重复应用实施例1〜12,有以下不同点:只喷施一次。 [0076] Application Example 1~12 repeated with the following differences: spraying only once.

[0077] 上述应用实施例的技术效果如图1〜图6所示。 Technical effect shown in [0077] As shown above applications embodiments 1 ~ 6.

[0078] 由图1可知，采用含不同浓度细胞分裂素的小桐子专用生长调节剂处理后，每个花序上的总花数均显著多于对照，其中以160mg/L为最佳浓度。 [0078] As shown in Figure 1, after containing different concentrations of cytokinin Jatropha special growth regulators, the total number of flowers on each inflorescence were significantly more than the control, which 160mg / L as the optimal concentration.

[0079] 由图2可知，采用含不同浓度细胞分裂素的小桐子专用生长调节剂处理后，雄花比例随细胞分裂素浓度的升高而降低，在320mg/L时达到最低；雌花和两性花的比例则随细胞分裂素浓度的升高而增加，均在320mg/L时达到最大。 After the [0079] Figure 2 shows, the use of Jatropha special growth regulators containing different concentrations of cytokinin, with the male proportion decreased cytokinin concentration increased, reaching the lowest in 320mg / L when; female and bisexual flowers The proportion increased with increasing cytokinin concentration, reached maximum at 320mg / L when.

[0080] 由图3可知，采用含细胞分裂素的小桐子专用生长调节剂处理后(右图)小桐子结果数量大大多于未处理的小桐子(左图)。 [0080] As shown in Figure 3, the use of Jatropha dedicated containing cytokinin growth regulators (right) the number of results Jatropha Jatropha much more than the untreated (left).

[0081] 由图4可知，采用含不同浓度细胞分裂素的小桐子专用生长调节剂处理后，每个果序的平均结果数量均显著多于对照，其中以160mg/L为最佳浓度。 [0081] Figure 4 shows, using post containing different concentrations of cytokinin Jatropha special growth regulators, the average number of results for each infructescence were significantly more than the control, which 160mg / L as the optimal concentration.

[0082] 由图5可知，采用含不同浓度细胞分裂素的小桐子专用生长调节剂处理后，每粒种子的平均重量均显著地略小于对照，在80mg/L时最小。 [0082] As shown in Figure 5, the use of containing different concentrations of cytokinin growth regulators Jatropha special treatment, the average weight of each seed were significantly slightly less than the control, the smallest in the 80mg / L when. [0083] 由图6可知，与对照相比，用含80mg/L或320mg/L细胞分裂素的小桐子专用生长调节剂处理后的小桐子种子含油率没有显著变化，但用含160mg/L细胞分裂素的小桐子专用生长调节剂处理小桐子后，其种子含油率显著高于对照。 [0083] Figure 6 shows, compared with the control, containing 80mg / L or 320mg / L of cytokinin Jatropha Jatropha seed oil content of special growth regulators after no significant changes, but containing 160mg / L Jatropha dedicated after cytokinin growth regulators Jatropha seed oil content was significantly higher than the control.