发明内容 DISCLOSURE

[0005] 本发明的目的，就是为了提供一种测定植物离体根比生长速率的装置和方法。 [0005] The object of the present invention is to provide a specific growth rate measured stumps apparatus and methods for plant away.

[0006] 为了达到上述目的，本发明采用了以下技术方案：一种测定植物离体根比生长速率的装置，包括顺序连接连通的离体根生物反应器、第一刻度管、虹吸管、废液瓶和第二刻度管；所述虹吸管的一端与第一刻度管的下部连接连通，虹吸管的另一端插入废液瓶内； 所述第二刻度管与废液瓶的下部连接连通。 [0006] To achieve the above object, the present invention adopts the following technical scheme: A method of determining in vitro plant roots than the growth rate of the device, including the order to connect the communication vitro root bioreactor, first graduated tube, siphon, waste liquid bottle and a second calibration tube; proximal end of the siphon tube is connected with the first graduation in communication, and the other end into the waste siphon bottle; the lower part of the second calibration tube connection communicating with the waste container.

[0007] 上述测定植物离体根比生长速率的装置，其中，所述第一刻度管上连接有带空气过滤器的通气管，所述废液瓶上连接有带空气过滤器的通气管。 [0007] the measurement vitro plant roots than the growth rate of the device, which is connected with the air filter on the first scale snorkel tube is connected with the air filter snorkel on the waste bottle .

[0008] 上述测定植物离体根比生长速率的装置，其中，所述第一刻度管的精度为0. 1毫升；所述第二刻度管的精度为1毫升。 [0008] The above assay in vitro plant roots than the growth rate of the device, wherein the first calibration precision tube is 0.1 ml; precision calibration of the second tube is 1 ml.

[0009] 一种用上述测定植物离体根比生长速率的装置测定植物离体根比生长速率的方法，包括如下步骤： [0009] An in vitro by the measurement of plant roots than the growth rate of unit vitro plant roots than the growth rate, comprising the step of determining:

[0010] 步骤一，取多份不同体积的植物离体根，分别浸泡到装有培养液的量筒中，根据排开的水的体积，得出每份植物离体根的体积； [0010] Step one, get multiple copies of different volumes vitro plant roots were soaked to the cylinder containing broth, depending on the volume of the displaced water volume obtained in vitro root of each plant;

[0011] 步骤二，在60°c下烘干植物离体根，称量得其干重； [0011] Step two, at 60 ° c under drying vitro plant roots, weighed obtaining dry weight;

[0012] 步骤三，以植物离体根的体积为横坐标，重量为纵坐标作图，并算出回归系数，即单位体积的植物离体根所对应的重量； [0012] Step three, in order to plant roots from the volume of the body as the horizontal, the vertical axis plotted weight, and calculate regression coefficients per unit volume of the plant from the root of the corresponding body weight;

[0013] 步骤四，把植物离体根样品接种到离体根生物反应器中培养，每天记录植物离体根排开水的体积； [0013] Step four, the plant root samples in vitro to in vitro root inoculation bioreactor culture, record volume vitro plant roots row water a day;

[0014] 步骤五，将植物离体根排开水的体积与步骤三算出的回归系数相乘，得出相应植物离体根的干重； [0014] Step five, the plants were excised roots discharge water volume and Step Three is calculated regression coefficient, and draw the corresponding vitro plant root dry weight;

[0015] 步骤六，作出生长曲线图； [0015] Step six, making growth chart;

[0016] 步骤七，结合生长曲线图，根据公另 [0016] Step seven, with growth chart, according to the public other

汁算出比生长速率， Specific growth rate was calculated juice,

[0017] 式中，X为终浓度，t为培养时间，X和X(l为接种浓度，μ为比生长速率； [0017] wherein, X is a final concentration, t is incubation time, X and X (l of inoculum concentration, μ is the specific growth rate;

[0018] 所述终浓度为培养结束时离体根的干重克数与培养液体积毫升数之比； [0018] The final concentration of the number of grams of dry weight of roots at the end of incubation in vitro with culture fluid volume (ml) ratio;

[0019] 所述接种浓度为接种离体根的干重克数与培养液体积毫升数之比。 [0019] The inoculum concentration from root dry weight in grams of body fluid volume and culture than the number of milliliters vaccination.

[0020] 本发明测定植物离体根比生长速率的装置在工作时，是先将该装置按顺序连接到离体根生物反应器上，再把植物离体根接种到生物反应器内，进行培养。 [0020] The present invention was measured in vitro plant roots than the growth rate of the device at work, is to order the device is connected to the body from the root of the bioreactor, and then plant in vitro root inoculation into the bioreactor, performed bring up. 随着离体根体积的增加，离体根生物反应器里的液体被不定根增长的体积排开到第一刻度管中，当第一刻度管中的液体高度与虹吸管顶端平齐时，根据虹吸原理，第一刻度管与虹吸管中的培养液会全部流入废液瓶中，然后，离体根生物反应器里的液体再随不定根增长排到第一刻度管中。 With the increase in vitro root volume, the volume in vitro root bioreactor liquid is scheduled to open adventitious roots growing into the first graduated tube, when the first calibration tube liquid level and siphon flush with the top, According to siphon principle, first graduated tube and siphon the broth will be fully into the waste bottle, and then, in vitro root bioreactor liquid and then discharged with adventitious roots grow first graduated tube. 最终离体根体积增加的量，就等于第二刻度管中的液体指示值加上第一刻度管的液体指示值。 Isolated Root final volume increase of the amount, equal to the second liquid in the tube indicates the scale value plus the value of the first liquid indicates graduated tube. 记录好每天离体根体积增加的量，折算成离体根重量增加的量，最后画出其生长曲线， 就可根据公式算出比生长速率。 Record the daily in vitro root volume increased amount, converted into the amount of weight gain in vitro root, and finally draw the growth curve, specific growth rate can be calculated according to the formula.