| Publication number | CN1966660 A |
| Publication type | Application |
| Application number | CN 200610154678 |
| Publication date | May 23, 2007 |
| Filing date | Nov 20, 2006 |
| Priority date | Nov 20, 2006 |
| Also published as | CN100453635C |
| Publication number | 200610154678.0, CN 1966660 A, CN 1966660A, CN 200610154678, CN-A-1966660, CN1966660 A, CN1966660A, CN200610154678, CN200610154678.0 |
| Inventors | 骆其君, 严小军, 周洪, 马斌, 尤仲杰 |
| Applicant | 宁波大学 |
| Export Citation | BiBTeX, EndNote, RefMan |
| Referenced by (6), Classifications (10), Legal Events (6) | |
| External Links: SIPO, Espacenet | |
技术领域 Technical Field
本发明涉及一种雨生红球藻的培养装置，尤其是涉及一种大规模培养雨生红球藻和转化虾青素的装置及其方法。 The present invention relates to a rain raw red ball algae culture apparatus, particularly to a large-scale cultivation of rain pluvialis astaxanthin and conversion apparatus and method.
背景技术 Background
雨生红球藻(Haematococcus pluvialis)是一种单细胞绿藻，其增殖依赖于细胞分裂，该藻在生长的特定阶段能够大量积累虾青素(Astaxanthin)。 Pluvialis algae (Haematococcus pluvialis) is a single-cell green algae, whose proliferation is dependent on cell division, which at certain stages of the growth of algae can accumulate a lot of astaxanthin (Astaxanthin). 虾青素具有良好的着色性能和极强的抗氧化性能，目前主要应用在水产养殖中，可以作为鲑鳟鱼类的饲料着色剂和添加剂，以改善鲑鳟鱼类的生长与生理功能；同时也是人类食品和保健品的优良添加剂，能够提高免疫力，抑制癌症。 Astaxanthin has good coloring performance and strong antioxidant properties, mainly used in aquaculture can be used as feed additives, coloring agents and salmon trout, and to improve the growth and physiological function of salmon and trout; and also the human food and excellent health care products additives, can enhance immunity, inhibit cancer. 因此，在医药方面的应用也具有很大的潜力。 Thus, in the application of medicine also has great potential.
由此可知，雨生红球藻是具有很好的开发前途的经济微藻。 It can be seen, the rain pluvialis is a good development prospects of economic microalgae.
雨生红球藻广阔的市场前景已引起了国内外的普遍关注，目前国外利用雨生红球藻生产天然虾青素的技术已取得长足发展，瑞典已有公司采用人工光源照射生物反应器进行生产。 Pluvialis algae broad market prospect has attracted widespread attention at home and abroad, in foreign countries, the use of rain pluvialis astaxanthin production of natural technology has made considerable progress, the Swedish company has been using artificial light source bioreactors produce. 美国Aquasearch公司在计算机控制的室外封闭生物反应器中进行培养，而Cyanotech公司则采用封闭的“平台”式生物反应器进行培养。 US Aquasearch companies in the computer-controlled outdoor closed bioreactors were cultured and Cyanotech companies adopt closed "platform" type of bioreactor culture. 目前国内有不少大专院校和科研院所对培养及提取天然虾青素等方面进行了研究，取得了不少的研究成果，如2005年10月12日公开的200510018203.4号中国发明专利申请公开说明书中公开了一种雨生红球藻高密度培养的气升式生物反应器，包括光生物反应器、水处理装置、光照明装置和气体供应装置，其特征在于水处理装置位于生物反应器外部，光照明装置位于生物反应器侧面，光照明装置包括内置源和外置源，温度控制装置位于生物反应器底部，在温度控制装置上是气体供应装置，采样排气孔位于反应器顶部内侧，藻液收集口位于生物反应器底部。 At present, there are many universities and research institutes for culture and extraction of natural astaxanthin and other aspects of the research, made a lot of research, Chinese patent application No. 200510018203.4 as October 12, 2005, discloses the specification discloses a rain pluvialis high density culture of airlift bioreactor, including the photobioreactor, water treatment devices, lighting equipment and a gas supply means, characterized in that the water treatment plant is located in a bioreactor exterior lighting means are located on the side of the bioreactor, the lighting device comprises a light source built-in and external sources, temperature control means is located at the bottom of the bioreactor, the temperature control means is a gas supply means, the sampling vent located inside top of the reactor alginate liquid collection ports at the bottom of the bioreactor. 而2004年12月22日公开的CN02138827.X号的中国发明专利则公开了一种培养雨生红球藻生产虾青素的方法，包括培养基配方、一步法生产工艺、培养基的循环使用和用二氧化碳调节培养液pH值诱导红球藻孢子的形成和虾青素积累的方法，雨生红球藻的营养细胞的生长、孢子转化和虾青素累积是在同一个生物反应器、同一培养基中完成，通过调控培养液的pH值促进孢子转化和虾青素累积，培养周期为12～15天，培养基经过回收—处理—重新配制，可以循环使用至少6次。 The number of Chinese patent CN02138827.X December 22, 2004 discloses a method disclosed is a culture of rain pluvialis astaxanthin, including media formulations, one-step production processes, recycling medium and culture pH adjustment with carbon dioxide method of inducing Haematococcus spore formation and accumulation of astaxanthin, pluvialis algal growth of vegetative cells, spores transformation and astaxanthin accumulation is in the same bioreactor, same the medium is completed, the pH of the culture was promoted by regulating the conversion of spores and astaxanthin accumulation, culture period of 12 to 15 days, the medium through recycling - Processing - reconstituted, can be recycled at least six times.
但是目前这些研究成果多数还是处在小型实验期内，在人工建成的水池中进行开放式培养，容易被其他浮游动植物或微生物所污染，不能形成大规模生产，因此与商品化生产之间还有一段距离。 But now most of these studies or in small experimental period, conduct an open culture in artificial pond built, easy to be contaminated plankton or other microorganisms, can not form a large-scale production, and commercialization of production and therefore also between some distance. 在实际生产中，目前虽有采用管道式生物反应器或小型固定化系统组成的生物反应器或气升式生物反应器进行培养雨生红球藻，其存在的缺陷是在较大规模生产条件下，要增加大量控制温度的设备投入，生产成本将大大增加。 In actual production, although the current use of piped bioreactor or small immobilization system consisting of bio-reactor or airlift bioreactor culture pluvialis algae, its shortcomings are in large-scale production conditions under controlled temperature to increase the number of equipment investment, production costs will be greatly increased. 在单位空间利用上，增加管道式生物反应器的管道，需极大增加建造的成本，加上藻细胞粘连遮蔽，导致培养期间透光性下降，引起管道壁清洗困难，只能适宜小规模生产。 The unit space utilization, increase pipeline conduit bioreactor required significantly increase the cost of construction, plus the algal cell adhesion shielding, resulting in a translucent decreased during the culture, causing difficulties in cleaning the pipeline wall, only suitable for small-scale production .
目前，尚未有利用自然水体，尤其是利用自然界水体来进行大规模培养雨生红球藻及获取虾青素的生产方法，其中主要的原因是雨生红球藻培养时期需要常温、弱光、高营养盐，而转化产生虾青素则需要在高温、强光、低营养盐的条件下进行。 At present, yet there is the use of natural water bodies, especially the use of natural water bodies to carry out large-scale cultivation of rain pluvialis and obtain astaxanthin production methods, the main reason is pluvialis algal culture period required room temperature, low light, high nutrients, and converted to produce astaxanthin is required at high temperature, light, low nutrient conditions. 即雨生红球藻培养与雨生红球藻转化和积累虾青素是处在两个不同的阶段，需要不同的环境条件。 That pluvialis algal culture and rain pluvialis astaxanthin transfer and accumulation are at two different stages, require different environmental conditions. 因此，在这两个阶段通常需要分别采用不同的生物反应器和不同的培养液，在不同的环境条件下进行，这种方法通常称之为“两步法”生产。 Thus, in these two stages typically requires using different bioreactors and different medium, carried out under different environmental conditions, this method is usually referred to as "two-step" production.
发明内容 DISCLOSURE
本发明所要解决的技术问题是提供一种大规模培养雨生红球藻与转化虾青素的装置及其方法，能够解决培养过程中培养与转化所需要温度与光照的矛盾，既保障雨生红球藻迅速生长，产生足够的生物量，又能够保证已经培养的雨生红球藻能够迅速转化与积累虾青素。 Technical problem to be solved by the present invention is to provide a large-scale cultivation of rain pluvialis astaxanthin and conversion apparatus and method capable of resolving contradictions. The cultivation and transformation of the required temperature and light, both to protect the rain of Health Haematococcus grow rapidly, produce enough biomass, but also to ensure that it had cultured pluvialis algae can be quickly converted and accumulated astaxanthin.
本发明解决上述技术问题所采用的技术方案为：一种大规模培养雨生红球藻和转化虾青素的装置，它包括固定架和设置在所述的固定架上的光生物反应器系统、充气装置、培养液灌输装置和静细胞收集装置，所述的光生物反应器系统由至少二个单元光生物反应器串接组合而成，所述的充气装置和所述的培养液灌输装置通过软管与所述的光生物反应器系统中最前面的第一单元光生物反应器的前端连接，所述的静细胞收集装置通过软管与所述的光生物反应器系统中的最后面的第二单元光生物反应器末端连接，所述的固定架上设置有浮沉控制装置。 The present invention solves the above problems is the technology used in the program: one large-scale cultivation of rain pluvialis astaxanthin and conversion apparatus comprising a holder and a holder disposed on the optical bioreactor system , inflatable devices, broth infusion device and static cell collection device, said light bioreactor system combination consisting of at least two cells in series photobioreactor culture medium of the inflator and the indoctrination device the front end is connected by a hose with said photo bioreactor system photo bioreactor foremost first unit, the static cell collection means through the photo bioreactor system with the hose in the rearmost second unit photobioreactor end connector is provided with the ups and downs of the control device on the holder.
所述的浮沉控制装置由设置在固定架上的浮子和坠子组成。 Drifting control means provided on the holder by a float and sinker components.
所述的单元光生物反应器之间可以设置有紧急开关。 The unit may be provided between the photo bioreactor emergency switch.
所述的单元光生物反应器可以由硬质或柔性材料制成，各单元光生物反应器之间可以通过软管相互串接。 The photo bioreactor unit may be made of a rigid or flexible material, the hose can be connected in series with each other between the respective unit light through bioreactor.
所述的第一单元光生物反应器和所述的第二单元光生物反应器之间可以设置有培养液循环管道。 Means between said first and second photo bioreactor bioreactor the optical unit may be provided with a culture solution circulation pipe.
所述的充气装置由气泵和酸性液体容器组成，所述的气泵通过软管与所述的酸性液体容器连接，所述的培养液灌输装置由第一水泵和培养液罐组成，所述的第一水泵通过软管与所述的培养液罐连接，所述的第一水泵和所述的气泵通过软管分别与所述的第一单元光生物反应器连接。 The inflatable device consists of an air pump and an acidic liquid container composition, said acidic liquid pump connected by a hose to said container, said broth infusion device comprises a first water pump and tank culture composition, said first a culture liquid tank through the pump and the hose connection, said first pump and said pump is connected to the first bioreactor unit light through a hose, respectively.
所述的静细胞收集装置包括第二水泵和培养产物收集罐，所述的第二水泵的进口通过软管与所述的第二单元光生物反应器的底部连接，所述的第二水泵的出口通过软管与所述的培养产物收集罐相连接。 Said stationary means including a second pump cell collection and culture product imports collecting tank, the second pump is connected through a hose with the bottom of the second unit of the photo bioreactor, said second pump The product was collected by cultivating export hose connected to the tank.
使用上述的装置大规模培养雨生红球藻和转化虾青素的方法，它包括以下步骤：①通过所述的培养液灌输装置向所述的光生物反应器系统内灌输培养液，其中培养液中的氮含量为5～20mg/L、磷含量为0.5～2.0mg/L；②接种③通过所述的充气装置向所述的光生物反应器系统内连续充气，增大气体与培养液的混合；④通过调节所述的浮沉控制装置将整个培养装置设置在水场中适宜雨生红球藻生长的水层深度，环境的水温为15~25℃，环境的光照强度为1000～5000Lx；⑤当培养15天后，藻细胞的密度达到10～30万个/毫升时，通过调节所述的浮沉控制装置将整个培养装置提升至水场中适宜雨生红球藻转化和积累虾青素的水层深度，环境的水温为25～30℃，环境的光照强度为6000～25000Lx；⑥通过所述的充气装置向所述的光生物反应器系统内连续充气；⑦将沉积在所述的第二单元光生物反应器底部的静细胞收集到所述的静细胞收集装置内，然后进行干燥，得到大量的富含虾青素的静细胞。 It means using the large-scale cultivation of rain pluvialis astaxanthin and conversion method comprising the steps of: ① to light within the bioreactor system to instill the culture broth by liquid infusion device, wherein culture The nitrogen content was 5 ~ 20mg / L, the phosphorus content is 0.5 ~ 2.0mg / L; ② ③ inoculated by the inflator to the inflatable continuous light within the bioreactor systems, and increase the gas medium mix; pluvialis algae growth ④ water depth of drifting by adjusting the control device to set the entire culture device suitable for water field, temperature environment of 15 ~ 25 ℃, light intensity environment of 1000 ~ 5000Lx ; ⑤ when incubated for 15 days when the density of algal cells of 10 to 300,000 / ml, drifting by adjusting the control device will enhance the entire culture device to water fields suitable pluvialis algae conversion and accumulation of astaxanthin The water depth, water temperature environment of 25 ~ 30 ℃, ambient light intensity is 6000 ~ 25000Lx; ⑥ according to the photo bioreactor system continuously through the inflatable device said inflator; ⑦ will be deposited in said Static intracellular second unit photobioreactor collected in the bottom of the static cell collecting means, and then dried to obtain a large number of rich astaxanthin static cells.
所述的浮沉控制装置由设置在固定架上的浮子和坠子组成，培养装置设置的水层深度是通过调节所述的浮子或者所述的坠子实现的。 Drifting control means provided on the holder by a float and sinker composition, culture means the water depth is set by adjusting the float or the pendant realized.
所述的静细胞收集装置包括第二水泵和培养产物收集罐，所述的第二水泵的进口通过软管与所述的第二单元光生物反应器的底部连接，所述的第二水泵的出口通过软管与所述的培养产物收集罐相连接，所述的第二水泵将沉积在所述的第二单元光生物反应器底部的静细胞收集到所述的培养产物收集罐内。 Said stationary means including a second pump cell collection and culture product imports collecting tank, the second pump is connected through a hose with the bottom of the second unit of the photo bioreactor, said second pump Static cell culture product by collecting tank outlet and the hose is connected, the second pump will be deposited in the second bioreactor unit light collected at the bottom of said culture product collection tank.
与现有技术相比，本发明的优点在于整个培养装置由设置在固定架上的光生物反应器系统、充气装置、培养液灌输装置和静细胞收集装置组成，光生物反应器系统由至少二个单元光生物反应器串接组合而成，充气装置和培养液灌输装置通过软管与光生物反应器系统中最前面的第一单元光生物反应器的前端连接，静细胞收集装置通过软管与光生物反应器系统中的最后面的第二单元光生物反应器末端连接，固定架上设置有浮沉控制装置，可以不需建立人工培养池和其他设施，而利用自然水体进行大规模雨生红球藻培养，减少了投资，节约了成本，提高经济收益；由于在自然界大水体中进行雨生红球藻培养，水体环境稳定，水体范围大，水体交换快，为培养高质量的雨生红球藻和获取高质量的虾青素提供了环境保障；利用自然界的水体进行大规模雨生红球藻培养，由于其培养的温度、光照、水流速度等环境条件比较稳定，单元生物反应器之间相连，培养液相互流动，加之往生物反应器中连续充气，培养条件优越，使得雨生红球藻的生长与分裂增殖基本同步，加之适宜营养水平的培养，雨生红球藻的生长率达到25％，生物量增长快，一般3～5天能够增加一倍；而通过固定架上设置的浮沉控制装置，可以在同一水体中将光生物反应器系统升降到所需要的水层深度，较好地解决了雨生红球藻培养和转化虾青素这二个阶段需要不同温度、不同光照和不同营养条件这对矛盾，在同一光生物反应器系统中用同一培养液就可以连续进行培养和转化，将过去的二步法合并为一步法，简化了生产工序，减轻了劳动强度，其光生物反应器系统可以连续使用，节约了资源；使用本发明的装置和方法，雨生红球藻的生长与分裂增殖基本同步，使得培养细胞转化为静细胞同一阶段完成，雨生红球藻转化虾青素的转化率可达90％以上，虾青素的含量可以达到1.3％～2.1％，在各单元光生物反应器之间设置紧急开关，可以在特殊情况下(比如泄漏时)通过关闭紧急开关，以避免其他的单元光生物反应器继续泄漏。 Compared with the prior art, the advantages of the present invention is that the entire culture device by a photo bioreactor system, the inflatable device is provided on the holder, the broth infusion device and static cell collection device component, the photo bioreactor system consists of at least two photo bioreactor units in series combination, the inflatable device and the front end of the culture medium infusion means is connected via a hose with the photo bioreactor system photo bioreactor foremost first unit, the static cell collection means through the hose and photo bioreactor system of the second unit photobioreactor rearmost end connection is provided with drifting control device may need an artificial culture ponds and other facilities, and the use of natural water bodies on a large scale Yusheng holder Haematococcus culture, reduced investment, cost savings, increase economic benefits; as in nature large water bodies pluvialis algal culture, water environment is stable, large water bodies range, water exchange fast for the training of high-quality Yusheng pluvialis and obtain high-quality astaxanthin provides environmental protection; use of water for large-scale natural pluvialis algal culture, since the temperature of its culture, light, flow rate and other environmental conditions are relatively stable, the cell bioreactor connected between each other flowing medium, coupled to the bioreactor continuously aerated, cultured in good condition, so pluvialis algae growth and proliferation of basic synchronization, combined with appropriate nutrition level of culture pluvialis algae growth rate of 25%, biomass growth quickly, usually three to five days can be doubled; and through the ups and downs provided on the holder control device that can be in the same body of water photo bioreactor system lifting water to the desired depth , solves the rain pluvialis astaxanthin culture and transformation of these two stages require different temperatures, different light and different nutritional conditions. This contradiction, in the same light with the same bioreactor system can be continuously broth cultured and transformation that will last for the one-step two-step merger, simplifying the production process, reduce labor intensity, the photo bioreactor system can be used continuously, saving resources; use of the device and method of the present invention, the rain of Health growth and proliferation of Haematococcus basic synchronized such cultured cells into the same stage of completion of the static cell, rain pluvialis astaxanthin conversion conversion rate of 90% astaxanthin content can reach between 1.3% 2.1% between the unit light bioreactor An emergency switch, it can (such as leakage) continues to leak by closing the emergency switch, in order to avoid other units photobioreactor in exceptional circumstances.
附图说明 Brief Description
图1为本发明培养和转化装置的结构示意图。 Chart 1 of the present invention in a schematic view of culture and transformation equipment.
具体实施方式 DETAILED DESCRIPTION
以下结合附图实施例对本发明作进一步详细描述。 Example below in conjunction with the accompanying drawings of the present invention will be further described in detail.
实施例一：一种大规模培养雨生红球藻和转化虾青素的装置，它包括固定架(图未显示)和设置在固定架上的光生物反应器系统1、充气装置2、培养液灌输装置3和静细胞收集装置4，光生物反应器系统1由五个单元光生物反应器串接组合而成，单元光生物反应器由柔性的不透水聚乙烯材料制成，各单元光生物反应器之间通过软管相互串接，单元光生物反应器之间的软管上设置有紧急开关5，充气装置2由气泵21和酸性液体容器22组成，气泵21通过软管与酸性液体容器22连接，培养液灌输装置3由第一水泵31和培养液罐32组成，第一水泵31通过软管与培养液罐32连接，第一水泵31和气泵21通过软管分别与第一单元光生物反应器11连接，气泵21所产生的压缩气体通过软管进入酸性液体容器22中，酸性液体容器22上设置有不吸水网滤，将高压气体所产生的气泡阻拦，经过酸冼后的气体再通过一根软管进入光生物反应器系统1中与培养液混合，静细胞收集装置4包括第二水泵41和培养产物收集罐42，第二水泵41的进口通过软管与第二单元光生物反应器12的底部连接，第二水泵41的出口通过软管与培养产物收集罐42相连接，固定架上设置有由浮子和坠子组成浮沉控制装置(图未显示)。 Example one: one large-scale cultivation of rain pluvialis astaxanthin and conversion apparatus comprising the holder (not shown) and is provided on the holder's photo bioreactor system, the inflatable device 2, culture infusion fluid collection device 3 and the static cell device 4, the photo bioreactor system 1 consists of five unit light bioreactor in series combination, means photobioreactor impervious flexible polyethylene material, each of the unit light connected in series with each other between the bioreactor through a hose, is provided with an emergency switch 5, the inflatable device 2 by the air pump 21 and the liquid container 22 consisting of acidic hose unit light bioreactor between the pump 21 through the hose with the acidic liquid container 22 is connected, broth infusion device 3 by the first pump 31 and the culture tank 32, a first pump 31 is connected with the culture tank through a hose 32, 21, respectively, through the hose and the first unit of the first pump 31 and the pump 11 connection photobioreactor, compressed gas generated pump 21 through the hose into the acidic liquid container 22, the acidic liquid container set 22 have non-absorbent mesh filter, high-pressure gas bubbles generated by block, after pickling of gas through a hose into the photo bioreactor system 1 was mixed with the culture, the cells were collected static means 4 comprises a second water pump 41 and the culture product collection tank 42, a second pump inlet through the hose 41 and the second unit photo bioreactor bottom connection 12, the second pump outlet 41 through the hose and culture product collection tank 42 is connected to the fixed frame set by the float and sinker have composed drifting control device (not shown).
实施例二：其它结构与实施例一相同，不同之处在于单元光生物反应器由刚性的有机玻璃制成。 Example II: other structure is the same as Embodiment 1, except that the optical element made of a rigid bioreactor plexiglass.
实施例三：其它结构与实施例一相同，不同之处在于光生物反应器系统1由十个单元光生物反应器组成，单元光生物反应器是由柔性聚乙烯材料制成的，第一单元光生物反应器11和第二单元光生物反应器12之间设置有培养液循环管道(图未显示)，培养液在光生物反应器系统1内是循环流动的，能够提高充气的利用率。 Example III: Other embodiments of an identical structure, except that the photo bioreactor system 1 is composed of ten units composed photobioreactor, the photobioreactor unit is made of a flexible polyethylene material, a first unit 12 disposed between the photo bioreactor 11 and the second unit has a photobioreactor medium circulation pipe (not shown), the culture medium in the photo bioreactor system 1 is circulating, it is possible to improve the utilization of the inflator.
实施例四：使用上述实施例的装置进行大规模培养雨生红球藻和转化虾青素的方法，它包括以下步骤：①通过培养液灌输装置3向光生物反应器系统1内灌输培养液，其中培养液中的氮含量为10mg/L、磷含量为0.8mg/L；②接种；③通过充气装置2向光生物反应器系统1内连续充气，增大气体与培养液的混合；④通过调节设置在固定架上的浮子和坠子，将整个培养装置设置在水场中适宜雨生红球藻生长的水层深度，环境的水温为20℃，环境的光照强度为3000Lx；⑤当培养15天后，藻细胞的密度达到20万个/毫升时，通过调节设置在固定架上的浮子和坠子，将整个培养装置提升至水场中适宜雨生红球藻转化和积累虾青素的水层深度，环境的水温为28℃，环境的光照强度为20000Lx；⑥通过充气装置2向光生物反应器系统1内连续充气；⑦通过第二水泵41将沉积在第二单元光生物反应器22底部的静细胞收集到培养产物收集罐42内，然后进行干燥，得到虾青素。 Example 4: Use device according to an embodiment of the large-scale cultivation of rain pluvialis astaxanthin and conversion method comprising the steps of: ① infusion broth medium to light through the bioreactor system 1 infusion device 3 , wherein the aqueous medium in the nitrogen content of 10mg / L, the phosphorus content of 0.8mg / L; ② vaccination; ③ 2 by inflating device to continuously aerated photo bioreactor system 1, increasing the mixed gas and the culture medium; ④ By adjusting the settings on the holder of the float and sinker, the entire culture device set suitable for water field pluvialis algae growing water depth, water temperature environment of 20 ℃, light intensity environment for 3000Lx; ⑤ When the culture After 15 days, the density of algal cells to reach 200,000 when / ml, by adjusting the settings on the holder of the float and sinker, will enhance the entire culture device suitable for rain to water the field pluvialis astaxanthin transformation and accumulation of water depth, water temperature environment of 28 ℃, light intensity environment for 20000Lx; ⑥ through the inflatable device 2 to the photo bioreactor system continuously inflatable 1; ⑦ through the second pump 41 will be deposited in the second unit photobioreactor 22 Static cell culture product collected at the bottom of the collection tank 42, and then dried to give astaxanthin.
实施例五：使用上述实施例的装置进行大规模培养雨生红球藻和转化虾青素的方法，它包括以下步骤：①通过培养液灌输装置3向光生物反应器系统1内灌输培养液，其中培养液中的氮含量为5mg/L、磷含量为1.0mg/L；②接种；③通过充气装置2向光生物反应器系统1内连续充气，增大气体与培养液的混合；④通过调节设置在固定架上的浮子和坠子，将整个培养装置设置在水场中适宜雨生红球藻生长的水层深度，环境的水温为15℃，环境的光照强度为1000Lx；⑤当培养15天后，藻细胞的密度达到10万个/毫升时，通过调节设置在固定架上的浮子和坠子，将整个培养装置提升至水场中适宜雨生红球藻转化和积累虾青素的水层深度，环境的水温为25℃，环境的光照强度为6000Lx；⑥通过充气装置2向光生物反应器系统1内连续充气；⑦通过第二水泵41将沉积在第二单元光生物反应器22底部的静细胞收集到培养产物收集罐42内，然后进行干燥，得到虾青素。 Example five: Use the device of the embodiment will be large-scale cultivation of rain pluvialis astaxanthin and conversion method comprising the steps of: ① infusion broth medium to light through the bioreactor system 1 infusion device 3 , wherein the nitrogen content of the culture medium was 5mg / L, the phosphorus content is 1.0mg / L; ② vaccination; ③ apparatus 2 through the inflator to the inflatable continuous photo bioreactor system 1, the mixed gas is increased and the culture liquid; ④ By adjusting the settings on the holder of the float and sinker, the entire culture device set suitable for water field pluvialis algae growing water depth, water temperature environment of 15 ℃, light intensity environment for 1000Lx; ⑤ When the culture After 15 days, the density of algal cells to reach 100,000 when / ml, by adjusting the settings on the holder of the float and sinker, will enhance the entire culture device suitable for rain to water the field pluvialis astaxanthin transformation and accumulation of water depth, water temperature environment of 25 ℃, ambient light intensity is 6000Lx; ⑥ inflator 2 through photo bioreactor system to continuously inflatable 1; ⑦ pump 41 through the second deposition of the second unit light bioreactor 22 Static cell culture product collected at the bottom of the collection tank 42, and then dried to give astaxanthin.
实施例六：使用上述实施例的装置进行大规模培养雨生红球藻和转化虾青素的方法，它包括以下步骤：①通过培养液灌输装置3向光生物反应器系统1内灌输培养液，其中培养液中的氮含量为8mg/L、磷含量为0.5mg/L；②接种；③通过充气装置2向光生物反应器系统1内连续充气，增大气体与培养液的混合；④通过调节设置在固定架上的浮子和坠子，将整个培养装置设置在水场中适宜雨生红球藻生长的水层深度，环境的水温为25℃，环境的光照强度为5000Lx；⑤当培养15天后，藻细胞的密度达到30万个/毫升时，通过调节设置在固定架上的浮子和坠子，将整个培养装置提升至水场中适宜雨生红球藻转化和积累虾青素的水层深度，环境的水温为30℃，环境的光照强度为25000Lx；⑥通过充气装置2向光生物反应器系统1内连续充气；⑦通过第二水泵41将沉积在第二单元光生物反应器22底部的静细胞收集到培养产物收集罐42内，然后进行干燥，得到虾青素。 Example six: use of the apparatus of the above embodiment for large-scale cultivation of rain pluvialis astaxanthin and conversion method comprising the steps of: ① infusion broth medium to light through the bioreactor system 1 infusion device 3 , wherein the nitrogen content of the culture medium was 8mg / L, the phosphorus content is 0.5mg / L; ② vaccination; ③ apparatus 2 through the inflator to the inflatable continuous photo bioreactor system 1, the mixed gas is increased and the culture liquid; ④ By adjusting the settings on the holder of the float and sinker, the entire culture device set suitable for water field pluvialis algae growing water depth, water temperature environment of 25 ℃, light intensity environment for 5000Lx; ⑤ When the culture After 15 days, the density of algal cells to reach 300,000 when / ml, by adjusting the settings on the holder of the float and sinker, will enhance the entire culture device suitable for rain to water the field pluvialis astaxanthin transformation and accumulation of water depth, water temperature environment of 30 ℃, ambient light intensity is 25000Lx; ⑥ inflator 2 through photo bioreactor system to continuously inflatable 1; ⑦ pump 41 through the second deposition of the second unit light bioreactor 22 Static cell culture product collected at the bottom of the collection tank 42, and then dried to give astaxanthin.
在实施例四、五、六中，在完成所有的培养过程后，可以用培养液灌输装置3向光生物反应器系统1灌输清水或消毒液，利用充气装置2产生的气流与液流对光生物反应器系统1进行清洗，并用静细胞收集装置4将清水或消毒液抽干，使整个培养装置进入重复使用。 Light streams in a gas stream with the fourth embodiment, five, six, after completion of all the training process can be used to instil the culture medium photo bioreactor system means 3 instill water or disinfectant, use the inflatable device 2 production bioreactor system for cleaning, and with static cell collection device 4 will drain the water or disinfectant, the entire culture device enters reused.
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| International Classification | C12P23/00, C12N1/12, C12M3/00 |
| Cooperative Classification | Y02P20/582, C12M21/02, C12M23/06, C12M23/56 |
| European Classification | C12M23/56, C12M21/02, C12M23/06 |
| Date | Code | Event | Description |
|---|---|---|---|
| May 23, 2007 | C06 | Publication | |
| Jul 18, 2007 | C10 | Request of examination as to substance | |
| Jan 21, 2009 | C14 | Granted | |
| Sep 22, 2010 | ASS | Succession or assignment of patent right | Owner name: LIJIANG CHENGHAI BER BIOLOGICAL DEVELOPMENT CO., L Free format text: FORMER OWNER: NINGBO UNIVERSITY Effective date: 20100818 |
| Sep 22, 2010 | C41 | Transfer of the right of patent application or the patent right | |
| Sep 22, 2010 | COR | Bibliographic change or correction in the description | Free format text: CORRECT: ADDRESS; FROM: 315211 NO.818, FENGHUA ROAD, JIANGBEI DISTRICT, NINGBO CITY, ZHEJIANG PROVINCE TO: 674200 GUANJIA VILLAGE, DONGHU VILLAGE COMMITTEE, CHENGHAI TOWN, YONGSHENG COUNTY, LIJIANG CITY, YUNNAN PROVINCE |
